2 Day Online Hands-On Workshop Gene Expression Profiling and Pathway Enrichment Analysis

INTRODUCTION 

Gene Expression Profiling and Pathway Enrichment Analysis are essential tools in modern genomics, providing deep insights into the molecular mechanisms underlying various biological processes and diseases. By examining gene expression profiles, researchers can measure the activity levels of thousands of genes simultaneously, identifying which genes are upregulated or downregulated under specific conditions.

Gene expression profiling offer a snapshot of the cellular state, revealing how genes respond to internal and external stimuli. This information is crucial for understanding complex biological phenomena, such as cell differentiation, disease progression, and response to treatments. However, the sheer volume of data generated can be overwhelming, necessitating robust analytical methods to extract meaningful insights.

Pathway enrichment analysis addresses this challenge by mapping differentially expressed genes to known biological pathways. This process identifies pathways that are significantly impacted by changes in gene expression, providing a functional context for the observed gene expression profiles. By linking genes to pathways, researchers can pinpoint key regulatory networks and molecular mechanisms driving the observed changes.

APPLICATIONS OF GENE EXPRESSION PROFILING AND PATHWAY ENRICHMENT ANALYSIS

1. Cancer Research and Treatment: Gene expression profiles and pathway enrichment analysis are crucial in cancer research for identifying differentially expressed genes between tumor and normal tissues.
2. Drug Discovery and Development: In pharmacogenomics, gene expression profiling and pathway enrichment analysis are employed to understand how different drugs affect gene expression and biological pathways.
3. Understanding Genetic Diseases: Gene expression profiles and pathway enrichment analysis are instrumental in studying genetic disorders.
4. Immune Response Profiling: These tools are used to study the immune response to infections, vaccines, and autoimmune diseases.
5. Developmental Biology: Gene expression profiling and pathway enrichment analysis are applied to understand the complex processes of development and differentiation.

DIFFERENT TYPES OF ANALYSIS

1. Differential Gene Expression: Differential gene expression analysis involves comparing gene expression profiles between different conditions, such as diseased vs. healthy tissues or treated vs. untreated samples. 
2. Co-expression Network: Co-expression network analysis examines the correlation patterns between gene expression profiles across different samples to identify groups of genes that are co-regulated.  
3. Time-series Expression: Time-series expression analysis tracks changes in gene expression over time, providing insights into dynamic biological processes such as development, response to treatment.

TOPICS COVERED

• Install Necessary Packages
• Quality Control and Trimming
• Mapping Reads to Reference Genome
• Counting Reads
• Differential Expression Analysis in R
• Normalization and Visualization
• Hierarchical Clustering
• GO and KEGG Pathway Enrichment Analysis

TAKEAWAY FROM THE MASTERCLASS

• Introductory Documentation: An introductory theory document to help you better understand the subject will also be provided.
• Trainers Slide Deck: After the completion of the session complete access to the trainers slide deck will also be provided
• Access to Trainers Repo: We will also be providing a complete access to trainers repository so that you can use it as reference later
• 8+ Hours of Live Training: During the course of 3 days we will be have live8+ hourse of training sessions with the participants.
• Guided Hands-On Exercises: Hands-On exercises are a must to better learn any technology and be able to reproduce it later.
• Participation Certificate: A Participations certificate is a must after successfully completing the training as a sign of accomplishment.

EXPECTED OUTCOMES

• Identification of Key Biomarkers: One of the primary outcomes of gene expression profiles and pathway enrichment analysis is the identification of key biomarkers.
• Understanding Disease Mechanisms: This analysis helps elucidate the underlying molecular mechanisms of diseases by identifying differentially expressed genes.
• Discovery of Potential Drug Targets: Gene expression profiles and pathway enrichment analysis can uncover potential drug targets by identifying genes and pathways
• Gene Regulation & Interaction Networks: This knowledge is essential for developing strategies that target multiple genes or pathways simultaneously, improving the efficacy of treatments for complex diseases.

TERMS & CONDITIONS

1. All fee paid is not refundable so please read all the terms & conditions before making any payments. If you still have any doubts please contact us and confirm and then only make the payment.
2. Participants need to bring their registration tickets along with a valid Institutional ID, then only they will be allowed to attend the session. Please reach out to our team in case of any exceptions.
3. Please fill all your details in the form correctly as those details will  be used in your certificate as well.
4. Participants need to bring their own computer (laptop) system for the program.
5. The software tools and other required software tools will be provided from our side for the purpose of this program.
6. Participants need to reach the venue and report 30 minutes prior to the start of the sessions.
7. Participants need to wear masks all the time inside the premises and abide by the other rules at the premises. 
8. Particpants need to attend all the sessions in order to be eligible for getting the certificate.
9. Welcome email will be sent to all the participants with all the details related to the program. Please check your Inbox/Spam folder for the email. 
10. All the details of the software installations and how to prepare your system for the Program will be shared with all the participants in the Welcome Email itself.